More about determination of an unknown amino acid from titration essay this is a chemistry lab report on an acid-base titration experiment 2714 words | 11 pages.
Undergraduate biochemistry students should have great familiarity with titration curves these curves allow the prediction of protonation states, charges, and isoelectric points here we describe an experiment in which students identify four amino acids based on their titration behavior students make solutions of each unknown amino acid and monitor the change in ph upon adding aliquots of a. Experiment 11 used a titration curve to determine the identity of an unknown amino acid the initial ph of the solution was 196, and the pka's found experimentally were 20, 40, and 985.
Titration: identification of an unknown amino acid biochemistry abstract water is important substance for all living organisms the physical and chemical properties of water play a central role in biological structure and function of the organism.
The unknown amino acid was recognized as glutamic acid and it was determined based on its pka and pi value from the graph in the titration curve of the unknown amino acid, it can be observed that at the ph reading of 216, 103 and 44 the graph shows an inflection curve making it the pka values of the unknown sample.
Students make solutions of each unknown amino acid and monitor the change in ph upon adding aliquots of a strong base an undergraduate experiment for biochemistry cassidy m dobson, cassidy m, and nathan s winter the identification of amino acids by interpretation of titration curves: an undergraduate experiment for biochemistry. To identify the unknown amino acid its pka was used as a comparison to the known value of lysine, histidine, and glutamate the equivalence point of titration from the titration curve of the unknown amino acid was estimated.